Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
NEW ENGLAND BIOGROUP LUNA Fluorescent validation slide. For use with LUNA-FX7 automated cell counters for quality control in fluorescent mode. One year shelf life.
LUNA Fluorescent validation slide. For use with LUNA-FX7 automated cell counters for quality control in fluorescent mode. One year shelf life. The LUNA FL Validation Slide is a slide with pre-fixed fluorescence beads and in a limited expiration period. It allows you to validate fluorescence cell counting performance of the following instruments and can be used for daily quality control purpose.
Alkali Scientific Radiant™ Probe No-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Radiant™ Probe No-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Meridian Life Science Inhibitor-Tolerant qPCR Mix 5x, 2ML
Meridian’s universal Inhibitor-Tolerant qPCR is a 5x formulation ready-to-use quantitative PCR supermix, containing all components except primers, probes and templates for direct qPCR. It is designed for developing qPCR multiplex assays that requiremore room for sample, minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.
Roche Diagnostics KAPA LIBRARY AMP PRIMER MIX
KAPA LIBRARY AMP PRIMER MIX *This item is non returnable.
Alkali Scientific Radiant™ Probe Hi-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
Radiant™ Probe Hi-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
LUNA NANOTECH INC 8-Position Mini Magnetic Rack For 1.5 ML Tubes
- Convenient Mini format magnetic racks for fast separation of magnetic beads in 1.5 mL microcentrifuge tubes. These racks can be used with our PuroMAG Mini kits as well as various magnetic beads offered by Luna Nanotech and other manufactureres.
- Angled design brings the tube bottom closer to the magnet to reduce magnetic separation time.
- Removable magnet strip allows resuspension of magnetic beads without the need to take the tubes out of the rack.
LUNA NANOTECH INC Ngsprep Size Selection Magnetic Beads, 5 ML
PuroMAG™ NGSPrep Magnetic Beads, 5 mL
The PuroMAG™ NGSPrep Magnetic Beads are designed for fast and highly effective DNA clean-up after polymerase chain reaction (PCR, qPCR, ddPCR), microarrays, sequencing and other types of ezymatic reactions by removing unincorporated primers, dNTPs (deoxynucleotide triphosphates), salts, and other impurities. Combination of SPRI technology and paramagnetic feature of the beads allows for specific size selection of DNA fragments during the clean-up procedure with high recovery of the DNA product. This is crucial application in library preparation for various sequencing techniques, including next-generation sequencing (NGS).
Features
- Easy and fast clean-up of DNA PCR samples with efficient recovery of the product
- Highly efficient size selection of purified fragmented genome or PCR product for NGS library prep
- High throughput clean-up of 96/384 samples
- Can be used on
LUNA NANOTECH INC Plasmid Midiprep Kit, 20 Preps
PuroSPIN™ Plasmid MIDIprep Kit, 20 Preps
PuroSPIN™ Plasmid MIDIprep Kit is designed to provide simple and easy-to-use method for extraction and purification of plasmid DNA from cultured bacterial cells. The purification technology is based on plasmid binding to silica spin-columns, which allows efficient removal of genomic DNA, protein, salt and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified plasmid DNA product for downstream applications, such as transfection, enzymatic digestion, or PCR.
Features:
- PuroSPIN™ Plasmid MIDIprep Kit can be used to purify plasmid DNA from up to 100 mL of overnight bacterial culture.
- Extaction of up to 200 µg of plasmid DNA product.
- Purify plasmid DNA from large volumes of bacterial culture
- Rapi
Meridian Life Science Lyo-Ready qPCR Buffer w/o, 100ML
Lyo-Ready qPCR Buffer w/o Excipients, 4x is a glycerol-free qPCR reaction buffer, designed to deliver highly reproducible, accurate qPCR assay results under fast thermal cycling conditions. In order to produce lyophilized, ambient-temperature stable qPCR reagents, dNTP, MgCl2, glycerol-free Taq DNA polymerase (such as MDX011 or MDX015) and excipients must be added prior to lyophilization.
Meridian Life Science Fast qPCR Buffer 4x, 2500UL
Fast qPCR Buffer, 4x is a novel buffer system that has been designed to be combined with a high-quality hot-start Taq DNA polymerase such as MDX008, to deliver highly reproducible, accurate assay results under fast thermal cycling conditions, delivering excellent results in fast qPCR assays.
Meridian Life Science Lyo-Ready qPCR Mix, 5ML
Lyo-Ready qPCR Mix, 2x is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2 and lyo-excipients. It is ideal for developing room temperature, lyophilized, multiplex qPCR diagnostic tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times. It can be used as a wet mix or it can be lyophilized without impacting its performance post rehydration.
Meridian Life Science Lyo-Ready qPCR Mix, 1ML
Lyo-Ready qPCR Mix, 2x is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2 and lyo-excipients. It is ideal for developing room temperature, lyophilized, multiplex qPCR diagnostic tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times. It can be used as a wet mix or it can be lyophilized without impacting its performance post rehydration.